Embryo Transfer. Credit, BBC.

Embryo Transfer. Credit, BBC.

Embryo transfer (ET) refers to the process of assisted reproduction when embryos are placed into the uterus of a female with the intention of establishing a pregnancy.  The process can involve a number of different steps and technologies, but generally requires two main components: generating and then obtaining (flushing) the embryos from the donor female and transferring each embryo into a different female (surrogate). ET is used to exploit the genetics of the female in the same way that frozen semen is used to exploit the genetics of the male. Advances in embryo technology are extremely useful in species such as cattle and buffalo which typically produce less than 1 calf per year. Comparatively, with embryo transfer, 9-12 calves can be produced per year.[1]

The first calf produced experimentally through ET was born in 1951, but it took until the 1970’s for the first calf to be commercially produced in the UK. The commercial ET industry was developed in North America in the early 1970’s with the introduction of exotic European breeds of cattle. As ET technology developed and improved, embryo transplant became more commonplace and affordable. Primarily, ET is used for genetic improvement rather than for increasing the numbers of a particular breed or phenotype.[2]

Identical twins as a result of embryo splitting.

Identical twins as a result of embryo splitting.

There are two procedures presently available for production of embryos from donor females: 1) superovulation, followed by artificial insemination and then flushing the uterus to gather embryos; and 2) in vitro fertilisation (IVF) consists of the recovery of eggs from the ovaries of the female then maturing and fertilising them outside the body until they are ready for implantation in foster females.

Although this technology is not commercially available in developing countries, ET technology could provide opportunities for the conservation and the development of minor breeds.[3] ET research and genetic improvement of local breeds could be made available to smallholder farmers in developing countries, through National Agriculture Research Centres, provided the expertise and infrastructure are available. For example, researchers at the International Livestock Research Institute (ILRI) based in Nairobi, Kenya succeeded in breeding their first calf through in vitro embryo production (IVEP) in 2009.[4] The East Africa Semen and Embryo Transfer Association, with support from the World Bank East African Agricultural Productivity Project (EAAPP) offers a minimum ET package including the harvesting of embryos from 3 donors and a transfer to at least 5 surrogates for Ksh 200,000 (nearly US$2,000), prohibitively expensive for most livestock producers.[5]

Contribution to Sustainable Intensification

Embryo transfer has a number of distinct advantages: by using laboratory equipment it reduces pressure on ‎natural resources used during a natural breeding process; cattle genotypes and production environments can be matched more closely; and it theoretically could allow farmers to more efficiently use their herd by renting their best cows as donors and utilise their lower-quality ones as surrogates.[6] Although embryo production and transfer may not have dramatic effects on rates of genetic gain, it can have considerable increases in efficiency. Heifer replacement programmes take a long time, maintaining a large number of males can be costly and the commercial relevance of some breeds may decline. If ET became a routine operation through artificial insemination, for example, beef operations based on this system could become competitive with pig and poultry production in terms of efficiency of food utilisation.[7]

Benefits and limitations
Requirements for embryo transfer

Developing countries face a number of challenges to establishing successful animal breeding programmes such as inadequate infrastructure for performance testing due to small and varied herd sizes, communal grazing often interferes with implementing animal health programmes, and poor nutrition, especially in cattle, which leads to low reproductive efficiency. [8]  As the selection of the donor female is one of the most important components in successfully applying embryo transfer (ET), improved cattle nutrition is a fundamental prerequisite.[9] Highly experienced and trained staff, and capital investment in adequate facilities, equipment and drugs are also necessary. [10]

Reproductive rate

The principal benefit of embryo transfer (ET) is the possibility to produce several offspring from a female, just as artificial insemination produces many offspring from the same male. The potential for reproduction in cattle is enormous – each female calf is born with an estimated 150,000 eggs. Yet, the average reproductive rate of a cow is around 1 calf per year, producing around 8-10 calves during a lifespan.[11] With ET, the average reproduction of a cow can be increased ten-fold in a given year and five-fold or higher per lifetime.[12]  Increasing the reproductive rate of selected females also means that genetically outstanding animals can contribute more to breeding programmes.[13]

Strategies for drought recovery

Drought is a major risk for livestock morbidity and a recurring challenge to maintaining herd size particularly in dryland areas in developing countries. In developed countries, some livestock keepers are using embryo transfer (ET) as a means of risk management against drought or other disasters that may significantly reduce herd size. By placing embryos into ET programmes and freezing them for use in the event of a drought, this allows for regenerating stock of superior quality rapidly. This method may also be less expensive than repurchasing cattle. Further, less meritable but nonetheless reproductively sound females from the surviving herd can be used as surrogates.[14] Although this strategy for risk management remains unavailable to most in developing countries, if ET technology becomes cheaper and more accessible to smallholders, it could become part of a suite of solutions to improve resilience to drought, climate change and other factors affecting livestock mortality rates.

Cost

Transport of embryos is much cheaper than live animals. However, cost is still a prohibitive factor in the uptake of embryo transfer (ET) technology, both in developed and developing countries. In North America and Europe, about 1 out of 500 calves born throughout the 1980’s was from embryo transfer[15] but this has increased significantly – more than 1 million bovine embryos were made available globally for transfer in 2013.[16] Based on the latest available data, less than 2% of all embryo transfers occurred in Africa.[17] Equipment and supplies tend to be more expensive to acquire in developing countries due to transportation costs, import tariffs, and limited access to finance.[18] In addition to capital investment, one of the greatest costs to an ET programme are the surrogates as they need to be in the same stage of the estrous cycle of the donor, which can be challenging to synchronize, and costly if not managed properly.[19]

In vitro fertilization (IVF) of the embryos, however, can lower costs of an ET programme as it facilitates the recovery of a large number of embryos from a single female, reducing the cost per embryo and improving economies of scale for larger programmes.[20]  ET technologies can also be adapted to local conditions to manage costs. For example, dry ice and alcohol baths are just as effective as more advanced freezing techniques, and these are also less labour intensive processes. Similarly, equipment such as filters, disposable syringes and plastic dishes are not essential for isolating embryos, suggesting that innovation in the field is possible, but more applied research is needed.[21]

Income generating potential

Value addition can be gained through demand for embryo transfer (ET). For example, farmers keeping indigenous or inferior quality breeds, but of adequate reproductive capacity, can rent their cows for use as surrogates. Alternatively, farmers keeping superior breeds can sell their 100-150 ova per cow per month. This strategy can be used to improve household income and increase the attractiveness of farming, as has been demonstrated in Brazil with hope for application in Kenya.[22]

References
  1. [1] Taneja, VK 1999, ‘Chapter 5: Dairy Breeds and Selection’ in: eds. L Falvey & C Chantalakhana, Smallholder Dairying in the Tropics. International Livestock Research Institute (ILRI), Nairobi, Available from: <http://www.ilri.org/InfoServ/Webpub/fulldocs/SmHDairy/Toc.htm#Table of Contents> [2 June 2015].
  2. [2] Troxel, TR (no date), Embryo Transfer in Cattle, University of Arkansas Cooperative Extension Service Printing Services, Little Rock.
  3. [3] Philipsson, J, Rege, JEO, Zonabend, E & Okeyo, AM (no date),  Sustainable breeding programmes for tropical low- and medium input farming systems, Available from: <https://cgspace.cgiar.org/bitstream/handle/10568/3965/module3_v3.pdf?sequence=1> [2 July 2015]
  4. [4] International Livestock Research Institute (ILRI), 2009 ‘Improving cattle genetics with in vitro embryo production technology’ 11 November 2009, ILRI News Available from: <http://www.ilri.org/ilrinews/index.php/archives/857> [2 July 2015].
  5. [5]Business Daily 2013, ‘Technology promises to deliver calves for Kenyan farmers’ 27 February 2013, Business Daily, Corporate News. Available from: <http://www.businessdailyafrica.com/Corporate-News/-/539550/1705954/-/10149b5/-/index.html> [2 July 2015]
  6. [6] International Livestock Research Institute (ILRI), 2009 ‘Improving cattle genetics with in vitro embryo production technology’ 11 November 2009, ILRI News Available from: <http://www.ilri.org/ilrinews/index.php/archives/857> [2 July 2015].
  7. [7] International Livestock Research Institute (ILRI), 2009 ‘Improving cattle genetics with in vitro embryo production technology’ 11 November 2009, ILRI News Available from: <http://www.ilri.org/ilrinews/index.php/archives/857> [2 July 2015].
  8. [8] Rege, JEO 1996, ‘Biotechnology options for improving livestock production in developing countries, with special reference to sub-Saharan Africa’ in: eds. SHB Lebbie & E Kagwini, Small Ruminant Research and Development in Africa: Proceedings of the Third Biennial Conference of the African Small Ruminant Research Network, UICC, Kampala, Uganda, 5-9 December 1994, International Livestock Research Institute (ILRI), Nairobi.
  9. [9] Wessler, B 2011, ‘Cattle Breeding: Embryo transfer’ 10 August 2011, Drovers CattleNetwork Industry News, Available from: <http://www.cattlenetwork.com/cattle-resources/dna-profiling/Cattle-Breeding-Embryo-transfer-127480893.html> [2 July 2015].
  10. [10] Rege, JEO 1996, ‘Biotechnology options for improving livestock production in developing countries, with special reference to sub-Saharan Africa’ in: eds. SHB Lebbie & E Kagwini, Small Ruminant Research and Development in Africa: Proceedings of the Third Biennial Conference of the African Small Ruminant Research Network, UICC, Kampala, Uganda, 5-9 December 1994, International Livestock Research Institute (ILRI), Nairobi.
  11. [11] Troxel, TR (no date), Embryo Transfer in Cattle, University of Arkansas Cooperative Extension Service Printing Services, Little Rock.
  12. [12] Seidel, GE Jr & Seidel, SM 1991, Training manual for embryo transfer in cattle, Food and Agriculture Organisation of the United Nations (FAO), Rome.
  13. [13]Rege, JEO 1995, ‘Application of biotechnology in genetic improvement, characterization and conservation of livestock’ In: eds. K Dzama, FN Ngwerume & E Bhebhe, Proceedings of the international symposium on livestock production through animal breeding and genetics, 10-11 May 1995. Harare, Zimbabwe, University of Zimbabwe, Harare.
  14. [14] Collyer, G & Ashworth, S 2009, ‘Embryo Transfer Strategies for Drought Recovery’ Chinchilla Vet Services & Info, Available from : <http://www.chinchillavet.com.au/ServicesInfo/Cattle/EmbryoTransfer/tabid/18860/Default.aspx> [2 July 2015].
  15. [15]Rege, JEO 1995, ‘Application of biotechnology in genetic improvement, characterization and conservation of livestock’ In: eds. K Dzama, FN Ngwerume & E Bhebhe, Proceedings of the international symposium on livestock production through animal breeding and genetics, 10-11 May 1995. Harare, Zimbabwe, University of Zimbabwe, Harare.
  16. [16] Perry, G (ed) 2012, 2012 Statistics of Embryo Cellection and Transfer in Domestic Farm Anilams, International Embryo Transfer Society (IETS) Available from: <http://www.iets.org/pdf/comm_data/december2013.pdf> [2 July 2015].
  17. [17]Rege, JEO 1995, ‘Application of biotechnology in genetic improvement, characterization and conservation of livestock’ In: eds. K Dzama, FN Ngwerume & E Bhebhe, Proceedings of the international symposium on livestock production through animal breeding and genetics, 10-11 May 1995. Harare, Zimbabwe, University of Zimbabwe, Harare.
  18. [18] Rege, JEO 1996, ‘Biotechnology options for improving livestock production in developing countries, with special reference to sub-Saharan Africa’ in: eds. SHB Lebbie & E Kagwini, Small Ruminant Research and Development in Africa: Proceedings of the Third Biennial Conference of the African Small Ruminant Research Network, UICC, Kampala, Uganda, 5-9 December 1994, International Livestock Research Institute (ILRI), Nairobi.
  19. [19] Kunkel, JR (no date), ‘Embryo Transfer’ Dairy Integrated Reproductive Management, IRM-26, University of Vermont, Burlington.
  20. [20] Rege, JEO 1996, ‘Biotechnology options for improving livestock production in developing countries, with special reference to sub-Saharan Africa’ in: eds. SHB Lebbie & E Kagwini, Small Ruminant Research and Development in Africa: Proceedings of the Third Biennial Conference of the African Small Ruminant Research Network, UICC, Kampala, Uganda, 5-9 December 1994, International Livestock Research Institute (ILRI), Nairobi.
  21. [21] Rege, JEO 1996, ‘Biotechnology options for improving livestock production in developing countries, with special reference to sub-Saharan Africa’ in: eds. SHB Lebbie & E Kagwini, Small Ruminant Research and Development in Africa: Proceedings of the Third Biennial Conference of the African Small Ruminant Research Network, UICC, Kampala, Uganda, 5-9 December 1994, International Livestock Research Institute (ILRI), Nairobi.
  22. [22] Mutembei, HM, Tsuma, VT, Muasa, BT, Muraya, J & Erastus, RM 2015, ‘Bovine in-vitro embryo production and its contribution towards improved food security in Kenya’ African Journal of Food, Agriculture, Nutrition and Development vol. 15, no. 1, pp. 9722-9743.

Case Studies

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Case study 1: Breeding for trypanosomosis resistance, The Gambia
Cow suffering from trypanosomosis. Credit ILRI

Cow suffering from trypanosomosis. Credit ILRI

African tsetse fly-transmitted trypanosomosis (ATT), sometimes referred to as ‘sleeping sickness,’ is estimated to cost more than US$4billion in losses each year in agricultural income, kills 3 million livestock and infects up to 75,000 people.[1] Symptoms of the infection include anaemia, weight loss, lymph node complications, infertility, and abortion.  There is no vaccine for ATT and treatment drugs are expensive and may become ineffective as drug-resistance develops. Despite a century of research, ATT remains one of the world’s most serious livestock diseases.

An alternative method of control is being explored through marker-aided selection(MAS) and embryo transfer to breed trypanosomiasis tolerant cattle. A small number of indigenous African ruminant breeds are already considered to be `trypano-tolerant,’ in that when infected, the consequences are relatively minor. The most ‘trypano-tolerant’ cattle breed is the West African N’dama (Bos Taurus) otherwise known for their resistance to many tick-borne infections, adaptability to humid and dry climates, low milk production and flavourful low-fat meat.[2]

In 1983 with the assistance of the International Trypanotolerance Center (ITC) (since renamed the West African Livestock Innovation Centre WALIC) based in Banjul, The Gambia, embryos were collected from N’Dama cows and transferred into Kenya Boran cows by using multiple ovulation and embryo transfer. The Centre was able to breed 177 offspring that were genotyped and used in mapping for MAS.[3] The results suggest it is possible to produce hybrids that are more tolerant of trypanosomiasis than either parent. WALIC continues to employ a variety of breeding strategies and technologies to improve resistance to ATT as well as improve milk production in local breeds.

References
  1. [1] IRIN News (no date), Africa: Tsetse fly costs agriculture billions every year, Available from: <http://www.irinnews.org/report/84351/africa-tsetse-fly-costs-agriculture-billions-every-year> [8 July 2015].
  2. [2] International Livestock Research Institute (ILRI) 2009, ‘Ndama’, Animal Genetics Training Resource, Available from: <http://agtr.ilri.cgiar.org/index.php?option=com_content&view=article&id=119&Itemid=134> [8 July 2015].
  3. [3] Hanotte, O, Ronin, Y, Agaba, M, Nilsson, P, Gelhaus, A, Horstmann, R, Sugimoto, Y, Kemp, S, Gibson, J, Korol, A, Soller, M & Teale, A 2003, ‘Mapping of quantitative trait loci controlling trypanolorance in a cross of tolerant West African N’Dama and susceptible East African Boran cattleProceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 13, pp. 7443-7448.
Case study 2: Ol Pejeta Conservancy, Kenya
Boran cattle herd in Ol Pejeta Conservancy, Laikipia, Kenya.

Boran cattle herd in Ol Pejeta Conservancy, Laikipia, Kenya.

The Ol Pejeta Conservancy is a non-profit organization located in Kenya’s Laikipia County. In addition to boasting East Africa’s largest black rhino sanctuary, Ol Pejeta holds the largest single herd of Boran cattle in the world. With a herd size of 2,000 top quality Boran breeding cows, Ol Pejeta is at the forefront of breeding for improved beef production.[1] As a member of the Boran Cattle Breeders’ Society, Ol Pejeta as embraced breeding technologies such as artificial insemination (AI) and embryo transfer (ET).

Although Ol Pejeta has not used ET for its own breeding programmes, but it has exported 1,600 embryos from around 100 cows to South Africa. To comply with export regulations, Ol Pejeta relied on expertise from South Africa which cost more than US$1,000 per calf born. This proved to be the most costly aspect of the embryo transfer process given that a team of 1 vet and 3 technicians are able to flush 20 cows and freeze all their embryos in the span of just 1 day.

ET has a number of benefits, such as mitigating the transmission of viral diseases such as Foot and Mouth Disease which are endemic across Africa. Local ET vets are also growing in numbers who can provide services to smallholder farmers, but drugs and other equipment necessary for ET remain high and the results have thus far been poor. Whist ET will be important to large scale dairy farmers, to multiply their top performing breeds, AI remains the most affordable and accessible option for smallholders for improving milk production.  Ol Pejeta also provides improved breeds and semen at subsidized rates in addition to training for local communities through their ‘Linking Livestock Markets with Wildlife Conservation’ programme.

References
  1. [1] Ol Pejeta Conservancy 2015, Boran Genetics, Available from: <http://www.olpejetaconservancy.org/boran_cattle/genetics> [8 July 2015].
Case Study 3: East Africa Semen and Embryo Transfer Association
Holstein calf with her Boran surrogate mother.

Holstein calf with her Boran surrogate mother.

Kenya has one of the largest dairy industries in sub-Saharan Africa, with an estimated 3.8 million dairy cattle. Nearly 85% of all milk in Kenya is produced by smallholders, for whom diary production is a major source of income. The industry however suffers from low quality cows with poor milk yielding capacity. Embryo transfer (ET) technology – the process of impregnating a cow with an embryo of superior breeds – offers the possibility for farmers to produce up to 10 calves per year or get a milking cow in 2 years compared to the current 15 year waiting time in some cases.

Supported by the World Bank East African Agricultural Productivity Project (EAAPP), interested farmers can receive advice on the procedures involved in using ET along with a tailor-made programme and financing options from the East Africa Embryo and Semen Transfer Association. The cost however still remains prohibitive for most farmers. A minimum package that includes harvesting embryos from 3 donors and transferring them to at least 5 surrogates costs Ksh200,000 (nearly US$2,000). The association is encouraging farmers to partner or work with cooperatives to meet the financial requirements which hopefully will pay off in the long run since a calf of superior quality can be sold at Ksh150,000 (nearly US$1,500).[1]

References
  1. [1] Business Daily 2013, Technology promises to deliver calves for Kenyan farmers, 27 Febuary 2013, Available from: <http://www.businessdailyafrica.com/Corporate-News/-/539550/1705954/-/10149b5/-/index.html> [8 July 2015].

Room for Innovation

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